AHA-1 (T183) polyclonal antibody
Product Specifications
Background
AHA-1 stimulates the inherent ATPase activity of yeast and human HSP 90 and interacts with the cytoplasmic tail of vesticular stomatitis virus glycoprotein. AHA-1 regulates HSP 90 by influencing the conformational state of the “ATP lid” and consequent N-terminal dimerization. It is crucial for cell viability under non-optimal growth conditions when HSP 90 levels are limiting. AHA-1 is a cytosolic protein and may transiently interact with the endoplasmic reticulum. It can have an affect on one step in the endoplasmic to Golgi trafficking. AHA-1 is expressed in numerous tissues, including brain, heart, skeletal muscle and kidney, and at lower levels in liver and placenta. It is induced by heat shock and treatment with the HSP 90 inhibitor 17-demethoxygeldanamycin.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
AHSA1; AHA1; C14ORF3; Activator of 90 kDa heat shock protein ATPase homolog 1; HSPC322
Swiss Prot
O95433
Reactivity
Human
Immunogen
Synthetic peptide, corresponding to amino acids 151-200 of Human AHA-1.
Conjugation
Unconjugated
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 38 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
AHA-1 (T183) polyclonal antibody detects endogenous levels of AHA-1 protein.
Applications Notes
Western blot (WB) analysis of AHA-1 (T183) polyclonal antibody at 1:500 dilution Lane1:L02 whole cell lysate (40ug) Lane2:HEK293T whole cell lysate (40ug) Lane3:786-O whole cell lysate (40ug) Lane4:HepG2 whole cell lysate (40ug) Lane5:U87-MG whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
Curated Selection
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