Adducin γ (P452) polyclonal antibody

Product Specifications

Background

Adducins are a family of cytoskeleton proteins encoded by three genes (α, β and γ) . Adducin is a protein associated with the inner leaflet of the plasma membrane and is one of the proteins localized at the spectrin-actin junction of the membrane skeleton. The cortical actin cytoskeletal network is lost during apoptosis and adducins are central in the cortical actin network organization. Adducin α is a cytoskeletal protein involved with sodium-pump activity in the renal tubule and is associated with hypertension. The expression of Adducin α and Adducin γ is ubiquitous in contrast to the restricted expression of Adducin β. Adducin β is expressed at high levels in brain andhematopoietic tissues, such as bone marrow in humans, and spleen in mice.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

ADD3; ADDG; ADDL; Adducin 3 (gamma) ; Adducin 3; Adducin-like protein 70; Gamma-adducin; Adducinγ

Swiss Prot

Q9UEY8

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 420-471 of Human Adducin γ.

Conjugation

Unconjugated

Applications

WB IHC IF

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 82 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Adducin γ (P452) polyclonal antibody detects endogenous levels of Adducin γ protein.

Applications Notes

Western blot (WB) analysis of Adducin γ (P452) polyclonal antibody at 1:500 dilution Lane1:BV2 whole cell lysate (40ug) Lane2:PC12 whole cell lysate (40ug) Lane3:LO2 whole cell lysate (40ug) Lane4:A549 whole cell lysate (40ug) Lane5:HepG2 whole cell lysate (40ug)