Actinin-α3 (E25) polyclonal antibody

The spectrin gene family encodes a diverse group of cytoskeletal proteins that include spectrins, dystrophins and α-actinins. There are four tissue-specific α-actinins, namely α-actinin-1, α-actinin-2, α-actinin-3 and α-actinin-4, which are localized to muscle and non-muscle cells, including skeletal, cardiac and smooth muscle cells, as well as within the cytoskeleton. Each α-actinin protein contains one Actin-binding domain, two calponin-homology domains, two EF-hand domains and four spectrin repeats, through which they function as bundling proteins that can cross-link F-Actin, thus anchoring Actin to a variety of intracellular structures. Defects in the gene encoding α-actinin-4 are the cause of focal segmental glomerulosclerosis 1 (FSGS1), a common renal lesion characterized by decreasing kidney function and, ultimately, renal failure.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Actinin-α3 (E25) polyclonal antibody detects endogenous levels of actinin-α3 protein, this antibody does not cross-react with other α-actinin isoforms.

Western blot (WB) analysis of Actinin-α3 (E25) polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate Lane2:Mouse muscle tissue lysate Lane3:Rat muscel tissue lysate