CYP27A1 (E131) polyclonal antibody

CYP27A1 participates in the degradation of cholesterol to bile acids in both the classic and acidic pathways.[1] It is the initiating enzyme in the acidic pathway to bile acids, yielding oxysterols by introducing a hydroxyl group to the carbon at the 27 position in cholesterol. In the acidic pathway, it produces 27-hydroxycholesterol from cholesterol whereas in the classic or neutral pathway, it produces 3β-hydroxy-5-cholestenoic acid.It is also involved in the metabolism of vitamin D3. While CYP27A1 is present in many different tissues, its function in these tissues is largely uncharacterized. In macrophages, 27-hydroxycholesterol generated by this enzyme may be helpful against the production of inflammatory factors associated with cardiovascular disease.

Sterol 26-hydroxylase; mitochondrial; 5-beta-cholestane-3-alpha; 7-alpha; 12-alpha-triol 27-hydroxylase; Cytochrome P-450C27/25; Cytochrome P450 27; Sterol 27-hydroxylase; Vitamin D (3) 25-hydroxylase; CYP27A1; CYP27

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Western blot (WB) analysis of CYP27A1 (E131) pAb at 1:500 dilution Lane1:LO2 whole cell lysate (40ug) Lane2:HepG2 whole cell lysate (40ug) Lane3:A549 whole cell lysate (40ug) Lane4:PC12 whole cell lysate (40ug) Lane5:AML-12 whole cell lysate (40ug)