PAF65α (Q53) polyclonal antibody
Product Specifications
Background
In eukaryotic systems, the process of initiating transcription from protein-coding genes requires the presence of RNA polymerase II and a broad family of auxiliary transcription factors. TFIID is a general transcription factor that initiates preinitiation complex assembly through direct interaction with the TATA promoter element. Functioning as a multisubunit complex consisting of a small TATA-binding polypeptide and other TBP-associated factors (TAFs), TFIID mediates promoter responses to various transcriptional activators and repressors. TAF6L, also known as PAF65A, is a 622 amino acid nuclear protein that functions as part of the PCAF (p300/CBP-associated factor) histone acetylase complex.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L; PCAF-associated factor 65-alpha; PAF65-alpha; TAF6L; PAF65A
Swiss Prot
Q9Y6J9
Reactivity
Human, Mouse
Immunogen
Synthetic peptide, corresponding to amino acids 20-70 of Human PAF65α.
Conjugation
Unconjugated
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 68 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
PAF65α (Q53) polyclonal antibody detects endogenous levels of PAF65α protein.
Applications Notes
Western blot (WB) analysis of PAF65α (Q53) Antibody at 1:1000 dilution Lane1:HepG2 whole cell lysate (10ug) Lane2:HEK293T whole cell lysate (10ug) Lane3:A549 whole cell lysate (10ug) Lane4:EC9706 whole cell lysate (10ug) Lane5:The Lung tissue lysate of Mouse (40ug)
Host or Source
Rabbit
Available Sizes
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