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PAF65α (Q53) polyclonal antibody

Product Specifications

Background

In eukaryotic systems, the process of initiating transcription from protein-coding genes requires the presence of RNA polymerase II and a broad family of auxiliary transcription factors. TFIID is a general transcription factor that initiates preinitiation complex assembly through direct interaction with the TATA promoter element. Functioning as a multisubunit complex consisting of a small TATA-binding polypeptide and other TBP-associated factors (TAFs), TFIID mediates promoter responses to various transcriptional activators and repressors. TAF6L, also known as PAF65A, is a 622 amino acid nuclear protein that functions as part of the PCAF (p300/CBP-associated factor) histone acetylase complex.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L; PCAF-associated factor 65-alpha; PAF65-alpha; TAF6L; PAF65A

Swiss Prot

Q9Y6J9

Reactivity

Human, Mouse

Immunogen

Synthetic peptide, corresponding to amino acids 20-70 of Human PAF65α.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 68 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

PAF65α (Q53) polyclonal antibody detects endogenous levels of PAF65α protein.

Applications Notes

Western blot (WB) analysis of PAF65α (Q53) Antibody at 1:1000 dilution Lane1:HepG2 whole cell lysate (10ug) Lane2:HEK293T whole cell lysate (10ug) Lane3:A549 whole cell lysate (10ug) Lane4:EC9706 whole cell lysate (10ug) Lane5:The Lung tissue lysate of Mouse (40ug)

Host or Source

Rabbit

Available Sizes

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