CNOT7 (N19) polyclonal antibody

Product Specifications

Background

CNOT7 (CCR4-NOT transcription complex, subunit 7), also known as CAF1 (CCR4-associated factor 1), hCAF-1 or BTG1-binding factor 1, is a member of the CAF1 family. Localizing to the nucleus, CNOT7 is ubiquitously expressed and is believed to function as a transcription factor, playing a role in a wide variety of processes. CNOT7 functions as a component of the evolutionarily conserved CCR4-NOT complex, a multi-subunit complex that participates in transcription as well as mRNA degradation. CNOT7 and other subunits of the CCR4-NOT complex play a role in the regulation of nuclear hormone receptor activities. CNOT7 directly binds to and interacts with RXR∫, TOB1, TOB2, BTG1, BTG2 and BTG3. In addition, CNOT7 knockout mice are sterile and show an increase in bone mass, suggesting an important role for CNOT7 in spermatogenesis and as a suppressor of bone mass and BMP (bone morphogenetic protein) actions in osteoblasts.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

CCR4-NOT transcription complex subunit 7; BTG1-binding factor 1; CCR4-associated factor 1; CAF-1; Caf1a

Swiss Prot

Q9UIV1

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 10-54 of Human CNOT7

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)

Modification

Unmodification

Molecular Weight

~ 32 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

CNOT7 (N19) polyclonal antibody detects endogenous levels of CNOT7 protein.

Applications Notes

Western blot (WB) analysis of CNOT7 (N19) Antibody at 1:1000 dilution Lane1:Myla2059 whole cell lysate (40ug) Lane2:HEK293T whole cell lysate (40ug) Lane3:Hela whole cell lysate (40ug) Lane4:PC12 whole cell lysate (40ug) Lane5:3T3-L1 whole cell lysate (40ug)