ENX-2 (E192) polyclonal antibody

Product Specifications

Background

In Drosophila, the Polycomb (PcG) gene family encodes chromatin proteins that are required for the repression of homeotic loci in embryonic development. PcG proteins work in conjunction with the trithorax-group (trxG) proteins, which activate homeobox gene expression during embryonic development. ENX-1, a mammalian homolog of the Drosophila gene enhancer of zeste, is a PcG protein that is ubiquitously expressed during early embryogenesis and becomes restricted to the central and peripheral nervous systems and sites of fetal hematopoiesis during later development. In the adult, ENX-1 is restricted to specific sites, including spleen, testis and placenta. ENX-2 is another mammalian homolog of the Drosophila gene enhancer of zeste and contains one SET domain. The gene for human ENX-2 maps to chromosome 17q21. ENX-2 expression is ubiquitous in adult and fetal tissue, where it may aid in maintaining heterochromatin stability.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Histone-lysine N-methyltransferase EZH1; ENX2; Enhancer of zeste homolog 1; EZH1; KIAA0388

Swiss Prot

Q92800

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 162-208 of Human ENX-2.

Conjugation

Unconjugated

Related Pathways

Epigenetic Writers and Erasers of Histone H3

Applications

WB IHC IF

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 85 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

ENX-2 (E192) polyclonal antibody detects endogenous levels of ENX-2 protein.

Applications Notes

Western blot (WB) analysis of ENX-2 (E192) polyclonal antibody at 1:500 dilution Lane1:MCF-7 whole cell lysate (40ug) Lane2:A2780 whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:A549 whole cell lysate (40ug) Lane5:HepG2 whole cell lysate (40ug) Lane6:H9C2 whole cell lysate (40ug) Lane7:3T3-L1 whole cell lysate (40ug)