DGK-δ (S66) polyclonal antibody

Diacylglycerol kinases (DGKs) phosphorylate diacylglycerol (DAG) to produce phosphatidic acid. DAG and phosphatidic acid are lipids that act as second messengers in signaling cascades. DGK-α influences cell activation and secretion of lethal exosomes, which in turn control cell death. DGK-β is abundant in restricted brain regions such as the caudate putamen and olfactory tubercle. DGK-γ encodes full-length and truncated transcripts that are present in a range of human tissues, with greatest expression observed in retina. DGK-δ is most abundant in skeletal muscle. DGK-ε shows specificity for arachidonylcontaining diacylglycerol and is expressed predominantly in testis. DGK-θ is most abundant in the cerebellum and hippocampus. DGK-ι is present in brain and retina as a predominant transcript of more than 12 kb, including a long 3-prime untranslated region, with additional low abundance transcripts of 9.5 and 7.5 kb. DGK-η is closely related to DGK-δ. DGK-ζ is most abundant in brain and muscle. DGKs have structural motifs that play regulatory roles, and these motifs form the basis for dividing the DGKs into five subtypes.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Western blot (WB) analysis of DGK-δ (S66) pAb at 1:500 dilution Lane1:A549 whole cell lysate (40ug) Lane2:Hela whole cell lysate (40ug) Lane3:A2780 whole cell lysate (40ug) Lane4:PC12 whole cell lysate (40ug) Lane5:3T3-L1 whole cell lysate (40ug)