TDG (K90) polyclonal antibody

Product Specifications

Background

In the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. G/T mismatch-specific Thymine DNA Glycosylase (TDG) is a nuclear protein which corrects G/T mismatches to G/C pairs by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and the mispaired thymine. TDG also corrects a subset of G/U mispairs inefficiently removed by the more abundant uracil glycosylases. Retinoic acid receptors interact physically and functionally with TDG, enhancing the ability of the retinoid X receptor and the retinoid X receptor/retinoid acid receptor complex to bind to their response elements. TDG interacts with, and is covalently modified by, the ubiquitin-like proteins SUMO-1 and SUMO-2/3, resulting in a reduction of the DNA substrate and AP site binding affinity of TDG. This sumoylation is associated with a significant increase in enzymatic turnover in reactions with a G/U substrate and the loss of G/T processing activity.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

G/T mismatch-specific thymine DNA glycosylase; Thymine-DNA glycosylase; Short name=hTDG; Thymine DNA glycosylase; hTDG

Swiss Prot

Q13569

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 28-109 of Human TDG.

Conjugation

Unconjugated

Applications

WB IHC IF

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 46 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

TDG (K90) polyclonal antibody detects endogenous levels of TDG protein.

Applications Notes

Western blot (WB) analysis of TDG (K90) pAb at 1:1000 dilution Lane1:CT-26 whole cell lysate (40ug) Lane2:PC12 whole cell lysate (40ug) Lane3:HCT116 whole cell lysate (40ug) Lane4:HEK293T whole cell lysate (40ug) Lane5:Hela whole cell lysate (40ug)