MTA1 (K182) polyclonal antibody
Product Specifications
Background
MTA1 (metastasis-associated protein 1) is a component of the NURD (for nucleosome remodeling and histone deacetylation) complex, which is associated with ATP-dependent chromatin-remodeling and histone deacetylase activity. MTA1 functions in conjunction with other components of NURD to mediate transcriptional repression as it facilitates the association of repressor molecules with the chromatin. Structurally, MTA1 contains a single SH3-binding motif and a zinc finger domain, along with a region similar to the corepressor protein N-Cor. MTA1 is normally expressed at low levels in various tissues and is more highly expressed in testis. Overexpression of MTA1 correlates with tumor invasion and metastasis in various carcinomas including colorectal, gastrointestinal and breast carcinomas.
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Metastasis-associated protein MTA1
Swiss Prot
Q13330
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 153-202 of Human MTA1.
Conjugation
Unconjugated
Related Pathways
ATP-Dependent Chromatin Remodeling Complexes, DNA Methylation
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 80 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
MTA1 (K182) polyclonal antibody detects endogenous levels of MTA1 protein.
Applications Notes
Western blot (WB) analysis of MTA1 (K182) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate (10ug) Lane2:MCF-7 whole cell lysate (20ug) Lane3:HepG2 whole cell lysate (40ug) Lane4:H9C2 whole cell lysate (40ug) Lane5:CT26 whole cell lysate (40ug)
Host or Source
Rabbit
CAS Number
9007-83-4
Available Sizes
Curated Selection
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