MTA1 (K182) polyclonal antibody

Product Specifications

Background

MTA1 (metastasis-associated protein 1) is a component of the NURD (for nucleosome remodeling and histone deacetylation) complex, which is associated with ATP-dependent chromatin-remodeling and histone deacetylase activity. MTA1 functions in conjunction with other components of NURD to mediate transcriptional repression as it facilitates the association of repressor molecules with the chromatin. Structurally, MTA1 contains a single SH3-binding motif and a zinc finger domain, along with a region similar to the corepressor protein N-Cor. MTA1 is normally expressed at low levels in various tissues and is more highly expressed in testis. Overexpression of MTA1 correlates with tumor invasion and metastasis in various carcinomas including colorectal, gastrointestinal and breast carcinomas.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Metastasis-associated protein MTA1

Swiss Prot

Q13330

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 153-202 of Human MTA1.

Conjugation

Unconjugated

Related Pathways

ATP-Dependent Chromatin Remodeling Complexes, DNA Methylation

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 80 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

MTA1 (K182) polyclonal antibody detects endogenous levels of MTA1 protein.

Applications Notes

Western blot (WB) analysis of MTA1 (K182) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate (10ug) Lane2:MCF-7 whole cell lysate (20ug) Lane3:HepG2 whole cell lysate (40ug) Lane4:H9C2 whole cell lysate (40ug) Lane5:CT26 whole cell lysate (40ug)