ADAR2 (R510) polyclonal antibody
Product Specifications
Background
ADAR2, also designated adenosine deaminase, RNA-specific (RED1), RNA-editing enzyme 1, DRABA2, DRADA2, ADAR2α-L1, ADAR2α-L2 and ADAR2α-L3, mediates RNA editing by destabilizing RNA through deamination of adenosine to inosine. ADAR2 is responsible for pre-mRNA editing of the glutamate receptor subunit B by site-specific deamination of adenosines. It can modify its own pre-mRNA and generate new splice sites. Translocation of endogenous ADAR2 from the nucleolus to the nucleoplasm results in increased editing of endogenous ADAR2 substrates. Alternative splicing of this gene results in several transcript variants that may influence RNA editing. RNA editing involves the deamination of adenosines at specific sites, the result of which can be a change in the amino acid sequence of the protein so that it differs from that predicted by the sequence of the DNA.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
ADARB1; RNA-editing deaminase 1; ADAR2; DRADA2; RED1; dsRNA adenosine deaminase; Double-stranded RNA-specific editase 1
Swiss Prot
P78563
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 480-530 of Human ADAR2.
Conjugation
Unconjugated
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 82 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
ADAR2 (R510) polyclonal antibody detects endogenous levels of ADAR2 protein.
Applications Notes
Western blot (WB) analysis of ADAR2 (R510) pAb at 1:1000 dilution Lane1:L02 whole cell lysate (20ug) Lane2:H9C2 whole cell lysate (40ug) Lane3:CT26 whole cell lysate (40ug) Lane4:MEF whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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