Ephrin-A3 (E196) polyclonal antibody

Product Specifications

Background

The Eph subfamily represents the largest group of receptor protein kinases identified to date. There is increasing evidence that they are involved in central nervous system function and in development. Ligands for Eph receptors include ephrin-A1 (LERK-1/B61), identified as a ligand for the EphA2 (Eck) receptor; ephrin-A2 (ELF-1), identified as a ligand for the EphA3 and EphA4 (Sek) receptors; ephrin-A3 (LERK-3), identified as a ligand for EphA5 (Ehk1) and EphA3 (Hek) receptors; ephrin-A4 (LERK-4), identified as a ligand for the EphA3 receptor; ephrin-A5 (AL-1), identified as a ligand for EphA5 (REK7) ; ephrin-B1 (LERK-2), identified as a ligand for the EphB1 (Elk) and EphB2 (Cek5) receptors; ephrin-B2 (LERK-5), identified as a ligand for the EphB1, EphB3 (Cek10) and EphB2 receptors; and ephrin-B3 (LERK-8), identified as a ligand for EphB1.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Ephrin A3; EFL-2; EHK1 ligand; EHK1-L; EPH-related receptor tyrosine kinase ligand 3; LERK-3; EFNA3; EFL2; EPLG3; LERK3

Swiss Prot

P52797

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 180-230 of Human Ephrin-A3.

Conjugation

Unconjugated

Applications

WB IHC IF

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 38 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Ephrin-A3 (E196) polyclonal antibody detects endogenous levels of Ephrin-A3 protein.

Applications Notes

Western blot (WB) analysis of Ephrin-A3 (E196) pAb at 1:500 dillution Lane1:Hct116 whole cell lysate Lane2:SK-OVCAR3 whole cell lysate Lane3:The Brain tissue lysate of Mouse Lane4:The Brain tissue lysate of Rat