CLK2 (R15) polyclonal antibody
Product Specifications
Background
CLK2 (CDC-like kinase 2) is a 499 amino acid nuclear protein that contains one protein kinase domain and belongs to the Ser/Thr protein kinase family. Using ATP, CLK2 phosphorylates serine- and arginine-rich (SR) components of the spliceosomal complex, possibly playing a role in the control of RNA splicing. CLK2 exists as two alternatively spliced isoforms, designated short and long, and is encoded by a gene which maps to human chromosome 1.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Dual specificity protein kinase CLK2; CDC-like kinase 2; CLK2
Swiss Prot
P49760
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids N-terminus of Human CLK2.
Conjugation
Unconjugated
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 60 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
CLK2 (R15) polyclonal antibody detects endogenous levels of CLK2 protein.
Applications Notes
Western blot (WB) analysis of CLK2 (R15) pAb at 1:500 dilution Lane1:C6 whole cell lysate (40ug) Lane2:3T3-L1 whole cell lysate (40ug) Lane3:HuT78 whole cell lysate (40ug) Lane4:Hela whole cell lysate (30ug) Lane5:A375 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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