TFIIE-β (K183) polyclonal antibody
Product Specifications
Background
The general transcription factor TFIIE consists of two subunits, α and β, and plays essential roles in transcription.Structure-function studies indicate that TFIIE has three-winged helix (WH) motifs, with one in TFIIEα and two in TFIIEβ. by binding to the clamp region of RNA polymerase II, TFIIEα-WH promotes the conformational change that transforms the promoter-bound inactive preinitiation complex to the active complex.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Transcription initiation factor IIE subunit beta; TFIIE-beta; General transcription factor IIE subunit 2; GTF2E2; TF2E2
Swiss Prot
P29084
Reactivity
Human, Mouse
Immunogen
Synthetic peptide, corresponding to amino acids 153-213 of Human TFIIE-β.
Conjugation
Unconjugated
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 33 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
TFIIE-β (K183) pAb detects endogenous levels of TFIIE-β protein.
Applications Notes
Western blot (WB) analysis of TFIIE-β (K183) pAb at 1:500 dilution Lane1:SGC7901 whole cell lysate (40ug) Lane2:HCT116 whole cell lysate (40ug) Lane3:The Testis tissue lysate of Mouse (40ug)
Host or Source
Rabbit
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