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MZF-1 (D10) polyclonal antibody

Product Specifications

Background

Zinc finger genes encode metal-binding proteins are transcriptional regulators of other genes. Myeloid zinc finger 1 (MZF-1), also designated zinc finger protein 42, and transcription factor ZBP-89, also designated zinc finger protein 148, belong to the Krüppel C2H2-type zinc-finger protein family. The gene encoding for the MZF-1 protein maps to chromosome 19q13.43, while the gene encoding for ZBP-89 is localized on chromosome 3q21.2. These proteins are nuclear proteins involved in the regulation of transcriptional events. MZF-1 regulates transcription during hemopoietic development and plays a role in myeloid cell differentiation. It regulates the CD34 promoter in a tissue-specific manner. MZF-1 and FHL3 can form a complex of high molecular mass with other proteins in the nucleus. MZF-1 is induced by retinoic acid and is primarily expressed in differentiating myeloid cells.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Myeloid zinc finger 1; MZF-1; Zinc finger and SCAN domain-containing protein 6; Zinc finger protein 42; MZF1; MZF; ZNF42; ZSCAN6

Swiss Prot

P28698

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 5-55 of Human MZF-1.

Conjugation

Unconjugated

Applications

WB

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 82 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

MZF-1 (D10) pAb detects endogenous levels of MZF-1 protein.

Applications Notes

Western blot (WB) analysis of MZF-1 (D10) pAb at 1:500 dilution Lane1:MG63 whole cell lysate (30ug) Lane2:Panc1 whole cell lysate (40ug) Lane3:C6 whole cell lysate (40ug) Lane4:3T3-L1 whole cell lysate (40ug) Lane5:K562 whole cell lysate (40ug)

Host or Source

Rabbit

Available Sizes

Frequently Asked Questions

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