ERK1/2 (R189) polyclonal antibody

The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at specific tyrosine and Threonine sites mapping within a characteristic Thr- Glu-Tyr motif. Phosphorylation at both the Thr and Tyr residues is required for full enzymatic activation. In response to activation, MAP kinases phos-phorylate downstream components on Serine and Threonine. Upstream MAP kinase regulators include MAP kinase kinase (MEK), MEK kinase and Raf-1. The ERK family has three additional members: ERK 3, ERK 5 and ERK 6.

Mitogen-activated protein kinase 3; MAP kinase 3; MAPK 3; ERT2; Extracellular signal-regulated kinase 1; ERK-1; Insulin-stimulated MAP2 kinase; MAP kinase isoform p44; p44-MAPK; Microtubule-associated protein 2 kinase; p44-ERK1; MAPK3; ERK1; PRKM3; Mitogen-activated protein kinase 1; MAP kinase 1; MAPK 1; ERT1; Extracellular signal-regulated kinase 2; ERK-2; MAP kinase isoform p42; p42-MAPK; Mitogen-activated protein kinase 2; MAP kinase 2; MAPK 2; MAPK1; ERK2; PRKM1; PRKM2

Immune Cell Signaling, Warburg Effect, Regulation of Microtubule Dynamics, T cell receptor signaling, Inhibition of Apoptosis, Insulin &Glucose Signaling, G Protein-coupled Receptors Signaling to MAPK Erk, MAPK Erk in Growth and Differentiation Pathway, Mitochondrial Control of Apoptosis, ErbB HER Signaling

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Western blot (WB) analysis of ERK1/2 (R189) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate (40ug) Lane2:U-87MG whole cell lysate (40ug) Lane3:HepG2 whole cell lysate (40ug) Lane4:C6 whole cell lysate (40ug) Lane5:MEF whole cell lysate (40ug)