ARP-1 (T7) polyclonal antibody

Product Specifications

Background

COUP (chicken ovalbumin upstream promoter) transcription factors have been cloned in several species and identified as orphan members of the steroid/thyroid hormone receptor superfamily. COUP-TFI (also designated COUP or EAR-3) and ARP-1 (also designated COUP-TFII) exhibit highly regulated and overlapping expression in most tissues. COUP-TFs are highly expressed in the developing and central nervous system, suggesting that these factors may be important in neural development and differentiation. COUP-TFs can compete for binding to response elements which are common to other members of this family, including RAR, RXR, PPAR, HNF-4, VDR and TR. They have been shown to act as negative regulators as well as initiators of transcription.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

COUP transcription factor 2; COUP-TF2; Apolipoprotein A-I regulatory protein 1; ARP-1; COUP transcription factor II; COUP-TF II; Nuclear receptor subfamily 2 group F member 2; NR2F2; ARP1; TFCOUP2; COUP TF2; COUPTF2

Swiss Prot

P24468

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to the N-terminal of Human ARP-1.

Conjugation

Unconjugated

Related Pathways

Nuclear Receptor Signaling

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 45 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

ARP-1 (T7) polyclonal antibody detects endogenous levels of ARP-1 protein.

Applications Notes

Western blot (WB) analysis of ARP-1 (T7) pAb at 1:500 dilution Lane1:MCF-7 whole cell lysate (40ug) Lane2:HEK293T whole cell lysate (20ug) Lane3:H9C2 whole cell lysate (20ug) Lane4:MEF whole cell lysate (40ug)