HisRS (E492) polyclonal antibody
Product Specifications
Background
Histidyl-tRNA synthetase (HisRS, Jo-1) is an aminoacyl-tRNA synthetase that charges transfer RNAs (tRNAs) with a cognate histidine residue to produce histidyl-tRNA. Histidyl-tRNA is essential for the incorporation of histidine into proteins. Systemic autoimmune diseases lead to aminoacyl-tRNA synthetase autoantibodies that are directed against self structures. HisRS is an antigen in systemic autoimmune diseases such as rheumatic arthritis or polymyositis with associated interstitial lung disease, thrombocytopenia and Raynaud’s phenomenon (vasospastic attacks that cause blood vessels to constrict) . HisRS autoantibodies are unlike anti-synthetase antibodies because they bind the nonlinear, exposed epitopes on native HisRS when the enzyme is complexed to tRNA (His) .
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Histidyl-tRNA synthetase; cytoplasmic; Histidine--tRNA ligase; HisRS; HARS; HRS
Swiss Prot
P12081
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 361-410 of Human HisRS.
Conjugation
Unconjugated
Applications
IHC IF
Dilution
IHC: 1:50~1:200 IF: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 60 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
HisRS (E492) polyclonal antibody detects endogenous levels of HisRS protein
Host or Source
Rabbit
Available Sizes
Curated Selection
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