HKR1 (W154) polyclonal antibody

Product Specifications

Background

Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc-finger proteins contain a Krueppel-type DNA binding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins. HKR1, also known as Krueppel-related zinc finger protein 1 or zinc finger protein 875, is a 659 amino acid nuclear protein that is thought to play a role in transcriptional regulation. Existing as two alternatively spliced isoforms, HKR1 is a member of the Krueppel C2H2-type zinc-finger protein family and contains thirteen C2H2-type zinc fingers and one KRAB domain. The gene encoding HKR1 maps to human chromosome 19q13.12.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Krueppel-related zinc finger protein 1; Protein HKR1; Zinc finger protein 875; HKR1; ZNF875

Swiss Prot

P10072

Reactivity

Humam

Immunogen

Synthetic peptide, corresponding to amino acids 111-160 of Human HKR1.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 73 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

HKR1 (W154) polyclonal antibody detects endogenous levels of HKR1 protein.

Applications Notes

Western blot (WB) analysis of HKR1 (W154) pAb at 1:1000 dilution Lane1:HEK293T whole cell lysate (20ug) Lane2:A549 whole cell lysate (40ug) Lane3:PC3 whole cell lysate (40ug)