Creatine Kinase M (Y14) polyclonal antibody

Product Specifications

Background

Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. The CKM isoform, predominant in skeletal muscle and heart tissue, is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. CKM reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

M-CK; CKMM; Creatine kinase M-type; Creatine kinase M chain; CKM; CK-M; MCK

Swiss Prot

P06732

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to the N-terminus of Human Creatine Kinase M.

Conjugation

Unconjugated

Applications

WB IHC IF

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 43 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Creatine Kinase M (Y14) polyclonal antibody detects endogenous levels of Creatine Kinase M protein.

Applications Notes

Western blot (WB) analysis of Creatine Kinase M (Y14) polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate Lane2:NIH-3T3 cell lysate Lane3:H9C2 cell lysate