Aldolase A (T9) polyclonal antibody
Product Specifications
Background
Fructose 1,6-bisphosphate aldolase catalyses the reversible condensation of glycerone-P and glyceraldehyde 3-phosphate into fructose 1,6-bisphosphate. Fructose 1,6-bisphosphate aldolase exists as three forms: the muscle-specific Aldolase A, the liver-specific Aldolase B, and the brain-specific Aldolase C. Aldolase A, B and C arose from a common ancestral gene from which Aldolase B first diverged. Aldolase A is one of the most highly-conserved enzymes known, with only about 2% of the residues changing per 100 million years. Aldolase B is regulated by the hormones insulin and glucagon, and has been implicated in hereditary fructose intolerance disease. Aldolase C is a polypeptide that is exclusively expressed in Purkinje cells. Aldolase C-positive Purkinje cells are organized in the cerebellum as stripes or bands that run from anterior to posterior across the cerebellum and alternate with bands of Aldolase Cnegative Purkinje cells.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Fructose-bisphosphate aldolase A; Lung cancer antigen NY-LU-1; Muscle-type aldolase; ALDOA; ALDA
Swiss Prot
P04075
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to the N-terminus of Human Aldolase A.
Conjugation
Unconjugated
Related Pathways
Warburg Effect
Applications
WB IHC IF
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 40 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Aldolase A (T9) polyclonal antibody detects endogenous levels of Aldolase A protein.
Applications Notes
Western blot (WB) analysis of Aldolase A (T9) pAb at 1:500 dilution Lane1:A549 whole cell lysate (40ug) Lane2:U-87MG whole cell lysate (40ug) Lane3:AML-12 whole cell lysate (40ug) Lane4:PC3 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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