Caper (R199) polyclonal antibody

Product Specifications

Background

Caper, also known as splicing factor HCC1 or hepatocellular carcinoma protein 1 and RNA binding region containing protein 2 (RNPC2), acts as a transcriptional coactivator for steroid nuclear receptors c-Jun, ERα and ERβ. Caper, a nuclear protein with highest concentrations in nuclear speckles, plays a role in the pre-mRNA splicing process. Two isoforms of Caper, HCC1.3 and HCC1.4, co-localize with pre-mRNA splicing factor SC35 and uridine-rich small nuclear RNAs. Caper is a widely expressed protein with highest levels detected in skeletal muscle, lung, brain and pancreas.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; Histone H3.2; Histone H3/m; Histone H3/o; HIST2H3A; HIST2H3C; H3F2; H3FM; HIST2H3D; Histone H3.3; 3F3A; H3.3A; H3F3; PP781; H3F3B; H3.3B; Histone H3.3C; Histone H3.5; H3F3C

Swiss Prot

O75787

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic methylpeptide derived from human Histone H3 around the methylation site of Lysine 80.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 39 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Caper (R199) pAb detects endogenous levels of Caper (R199) protein.

Applications Notes

Western blot (WB) analysis of Caper (R199) pAb at 1:500 dilution Lane1:C6 whole cell lysate (40ug) Lane2:3T3-L1 whole cell lysate (40ug) Lane3:HepG2 whole cell lysate (40ug) Lane4:U-87MG whole cell lysate (40ug) Lane5:HEK293T whole cell lysate (40ug)