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AURKB/C (K197) polyclonal antibody

Product Specifications

Background

Aurora kinases belong to a highly conserved family of mitotic serine/threonine kinases with three members identified among mammals: Aurora A, B, and C. Studies on the temporal expression pattern and subcellular localization of Aurora kinases in mitotic cells suggest an association with mitotic structure. Aurora kinase functional influences span from G2 phase to cytokinesis and may be involved in key cell cycle events such as centrosome duplication, chromosome bi-orientation and segregation, cleavage furrow positioning, and ingression.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Aurora kinase B; Aurora 1; Aurora- and IPL1-like midbody-associated protein 1; AIM-1; Aurora/IPL1-related kinase 2; ARK-2; Aurora-related kinase 2; STK-1; Serine/threonine-protein kinase 12; Serine/threonine-protein kinase 5; Serine/threonine-protein kinase aurora-B; AURKB; AIK2; AIM1; AIRK2; ARK2; STK1; STK12; STK5; Aurora kinase C; Aurora 3; Aurora/IPL1-related kinase 3; ARK-3; Aurora-related kinase 3; Aurora/IPL1/Eg2 protein 2; Serine/threonine-protein kinase 13; Serine/threonine-protein kinase aurora-C; AURKC; AIE2; AIK3; AIRK3; ARK3; STK13

Swiss Prot

Q96GD4/Q9UQB9

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 163-213 of Human AURKB.

Conjugation

Unconjugated

Related Pathways

Regulation of Microtubule Dynamics

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 35, 39 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

AURKB/C (K197) polyclonal antibody detects endogenous levels of AURKB and AURKC protein.

Applications Notes

Western blot (WB) analysis of AURKB/C (K197) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate (20ug) Lane2:HepG2 whole cell lysate (20ug) Lane3:PC3 whole cell lysate (40ug) Lane4:A549 whole cell lysate (40ug) Lane5:PC12 whole cell lysate (40ug) Lane6:AML-12 whole cell lysate (40ug)

Host or Source

Rabbit

Available Sizes

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