AMPKβ1 (V175) polyclonal antibody

Product Specifications

Background

AMPKβ1 is a member of the SNF1 kinase family. It is a heterotrimeric protein comprising of an alpha (catalytic), beta (non-catalytic) and gamma (non-catalytic) subunits, 63, 38 and 38kDa respectively.AMPKβ1 regulates fatty acid and sterol synthesis by phosphorylation of acteyl-coA. It also regulates cholesterol synthesis via phosphorylation and in-activation of hydroxymethylglutaryl-CoA reductase.Two isoforms of AMPKβ1 have been found.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

5'-AMP-activated protein kinase subunit beta-1; AMPK subunit beta-1; AMPKb; PRKAB1; AMPK; AMPK β1

Swiss Prot

Q9Y478

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 143-190 of Human AMPKβ1.

Conjugation

Unconjugated

Related Pathways

Hypoxia Signaling, AMPK Signaling Pathway, MTOR signaling

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 38 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

AMPKβ1 (V175) polyclonal antibody detects endogenous levels of AMPKβ1 protein.

Applications Notes

Western blot (WB) analysis of AMPKβ1 (V175) polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate Lane2:HEK293T cell lysate Lane3:NIH-3T3 cell lysate Lane4:PC12 cell lysate