AMPKβ1 (V175) polyclonal antibody
Product Specifications
Background
AMPKβ1 is a member of the SNF1 kinase family. It is a heterotrimeric protein comprising of an alpha (catalytic), beta (non-catalytic) and gamma (non-catalytic) subunits, 63, 38 and 38kDa respectively.AMPKβ1 regulates fatty acid and sterol synthesis by phosphorylation of acteyl-coA. It also regulates cholesterol synthesis via phosphorylation and in-activation of hydroxymethylglutaryl-CoA reductase.Two isoforms of AMPKβ1 have been found.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
5'-AMP-activated protein kinase subunit beta-1; AMPK subunit beta-1; AMPKb; PRKAB1; AMPK; AMPK β1
Swiss Prot
Q9Y478
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 143-190 of Human AMPKβ1.
Conjugation
Unconjugated
Related Pathways
Hypoxia Signaling, AMPK Signaling Pathway, MTOR signaling
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 38 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
AMPKβ1 (V175) polyclonal antibody detects endogenous levels of AMPKβ1 protein.
Applications Notes
Western blot (WB) analysis of AMPKβ1 (V175) polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate Lane2:HEK293T cell lysate Lane3:NIH-3T3 cell lysate Lane4:PC12 cell lysate
Host or Source
Rabbit
Available Sizes
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