PDCD4 (K453) polyclonal antibody

Product Specifications

Background

The transformation suppressor gene Pdcd-4 (programmed cell death gene 4) inhibits the tumor-promoter mediated transformation of mouse keratinocytes and is a potential tumor suppressor gene in the development of human lung cancer. Biochemical analysis suggests that the Pdcd-4 protein is involved in protein translation as well as in nuclear events. Pdcd-4 directly interacts with the RNA helicase eIF4A and inhibits protein synthesis by interfering with the assembly of the cap-dependent translation initiation complex. Pdcd-4 also suppresses the transactivation of AP-1 responsive promoters by c-Jun, suggesting that the transformation-suppressor activity of Pdcd-4 might be due, at least in part, to the inhibition of c-Jun activity. In addition to affecting c-Jun phosphorylation, Pdcd-4 blocks the recruitment of the co-activator p300 by c-Jun.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Programmed cell death protein 4; Neoplastic transformation inhibitor protein; Nuclear antigen H731-like; Protein 197/15a; PDCD4; H731

Swiss Prot

Q53EL6

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 422-474 of Human PDCD4.

Conjugation

Unconjugated

Related Pathways

Translational Contral elF4 and p70 S6 Kinase

Applications

WB IHC IF

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 51 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

PDCD4 (K453) polyclonal antibody detects endogenous levels of PDCD4 protein.

Applications Notes

Western blot (WB) analysis of PDCD4 (K453) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate (40ug) Lane2:HCT116 whole cell lysate (40ug) Lane3:A549 whole cell lysate (40ug) Lane4:PC12 whole cell lysate (40ug) Lane5:CT26 whole cell lysate (40ug)