C-Met (E999) polyclonal antibody
Product Specifications
Background
C-Met, a member of the tyrosine kinase superfamily, is the receptor for hepatocyte growth factor, also known as scatter factor (HGF/SF) . The mature c-Met protein is a disulfide-linked heterodimer with Mr=190 kDa composed of a heavily glycosylated alpha subunit that is completely extracellular in localization, and a beta subunit comprised of an extracellular ligand binding domain, a single transmembrane domain, and a cytoplasmic tyrosine kinase domain. Cells expressing c-Met include epithelial cells, endothelial cells, blood cells of various types, and glomerular mesenchymal cells.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Hepatocyte growth factor receptor; HGF receptor; HGF/SF receptor; Proto-oncogene c-Met; Scatter factor receptor; SF receptor; Tyrosine-protein kinase Met; MET
Swiss Prot
P08581
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 961-1010 of Human c-Met.
Conjugation
Unconjugated
Applications
WB IHC IF
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 156 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
C-Met (E999) polyclonal antibody detects endogenous levels of c-Met protein.
Applications Notes
Western blot (WB) analysis of c-Met (E999) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate (40ug) Lane2:A375 whole cell lysate (40ug) Lane3:SGC7901 whole cell lysate (40ug) Lane4:SK-OVCAR3 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
HV080113 100 µg
Human IgG1 (E233A/L235A), kappa Isotype Control Antibody (HyHEL-10)
Sign In for Pricing
View Details 
MBS2152382-01 0.1 mL
Monoclonal Antibody to Proteasome 26S Subunit, Non ATPase 10 (PSMD10)
Sign In for Pricing
View Details MBS2152382-02 0.2 mL
Monoclonal Antibody to Proteasome 26S Subunit, Non ATPase 10 (PSMD10)
Sign In for Pricing
View Details MBS2152382-03 0.5 mL
Monoclonal Antibody to Proteasome 26S Subunit, Non ATPase 10 (PSMD10)
Sign In for Pricing
View Details MBS2152382-04 1 mL
Monoclonal Antibody to Proteasome 26S Subunit, Non ATPase 10 (PSMD10)
Sign In for Pricing
View Details