Wnt-1 (G331) polyclonal antibody

Product Specifications

Background

Products of the highly conserved Wnt gene family play key roles in regulating cellular growth and differentiation. The prototype member of the Wnt gene family, Wnt-1, is a cysteine-rich secreted glycoprotein that associates with cell membranes and likely functions as a key regulator of cellular adhesion. β-catenin, a cadherin-binding cellular adhesion protein which also binds the tumor supressor gene APC, has been identified as a downstream target of a signal transduction pathway mediated by Wnt-1. Wnt-1 is essential for normal development of the embryonic nervous system and its expression is normally limited to the embryonic neural tube and adult spermatids. When improperly expressed in mammary tissue, Wnt-1 contributes to hyperplasia and tumorigenic progression. Wnt family members have been shown to interact with Sonic hedgehog (Shh) in vivo to induce myogenesis in somitic tissue.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Proto-oncogene Wnt-1; Proto-oncogene Int-1 homolog; WNT1; INT1; WNT 1

Swiss Prot

P04628

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 301-350 of Human Wnt-1.

Conjugation

Unconjugated

Applications

WB IHC IF

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 41 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Wnt-1 (G331) polyclonal antibody detects endogenous levels of Wnt-1 protein.

Applications Notes

Western blot (WB) analysis of Wnt-1 (G331) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate (40ug) Lane2:Hela whole cell lysate (40ug) Lane3:DLD whole cell lysate (40ug) Lane4:The Testis tissue lysate of Mouse (40ug) Lane5:The Testis tissue lysate of Rat (40ug)