SSB (T362) polyclonal antibody

Product Specifications

Background

Ro/SSA is a ribonucleoprotein that binds to autoantibodies in 35 to 50% of patients with SLE and in up to 97% of patients with Sjogren syndrome. The Ro/SSA particle consists of a single immunoreactive protein noncovalently bound with one of four small RNA molecules. Most anti-Ro/SSA-positive sera have antibodies not only against the immunoreactive protein, but also against an Ro/SSA protein. The genes which encode the two proteins map to human chromosomes 11p15.5 and 1q31, respectively. La/SSB is an autoimmune RNA-binding protein that plays a role in the transcription of RNA polymerase III was originally defined by its reactivity with autoantibodies from patients with Sjogren syndrome and SLE.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Autoantigen La; La; La autoantigen; La autoantigen homolog; La protein; La ribonucleoprotein; La ribonucleoprotein domain family member 3; LA; LARP3; Lupus La antigen; Lupus La protein; Lupus La protein homolog; MGC118101; MGC93380; mRNA for autoantigen; OTTMUSP00000014043; RP23 273G23.1; Sjoegren syndrome type B antigen; Sjoegren syndrome type B antigen; Sjogren syndrome antigen B (autoantigen La) ; Sjogren syndrome antigen B; SS B; SS-B; SS-B/La protein; SSB

Swiss Prot

P05455

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 331-380 of Human SSB.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~50 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

SSB (T362) polyclonal antibody detects endogenous levels of SSB protein.

Applications Notes

Western blot (WB) analysis of SSB (T362) pAb at 1:1000 dilution Lane1:A2780 whole cell lysate (20ug) Lane2:PC3 whole cell lysate (20ug) Lane3:L02 whole cell lysate (20ug) Lane4:The Ovary tissue lysate of Rat (40ug) Lane5:AML-12 whole cell lysate (40ug) Lane6:3T3-L1 whole cell lysate (40ug)