NFATc4 (N670) polyclonal antibody

Product Specifications

Background

NFATc4 (nuclear factor of activated T cells, cytoplasmic, calcineurin-dependent 4) is a member of the nuclear factors of activated T cells DNA-binding transcription complex that influences cytokine gene expression, cardiac hypertrophy and adipocyte differentiation. This complex consists of at least two components: a cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation and an inducible nuclear component. Other members of this family participate in the formation of this complex. NFATc4 plays a role in the inducible expression of cytokine genes in T cells, including the induction of IL-2 and IL-4. p38 MAP kinase phosphorylates multiple residues in the NFAT homology domain of NFATc4.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Nuclear factor of activated T-cells, cytoplasmic 4; NF-ATc4; NFATc4; T-cell transcription factor NFAT3; NF-AT3; NFATC4; NFAT3

Swiss Prot

Q14934

Reactivity

Human, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 635-689 of Human NFATc4.

Conjugation

Unconjugated

Related Pathways

AMPK Signaling Pathway

Applications

WB IHC IF

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 140 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

NFATc4 (N670) polyclonal antibody detects endogenous levels of NFATc4 protein.

Applications Notes

Western blot (WB) analysis of NFATc4 (N670) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate (40ug) Lane2:PC12 whole cell lysate (40ug) Lane3:A2780 whole cell lysate (40ug) Lane4:H1792 whole cell lysate (40ug)