Cyclin D1 (L283) polyclonal antibody
Product Specifications
Background
Considerable effort directed towards the identification of G1 cyclins has led to the isolation of cyclin D, cyclin C and cyclin E. Of these, cyclin D corresponds to a putative human oncogene, designated PRAD1, which maps at the site of the Bcl1 rearrangement in certain lymphomas and leukemias. Two additional human type D cyclins, as well as their mouse homologs, have been identified. Evidence has established that members of the cyclin D family function to regulate phosphorylation of the retinoblastoma gene product, thereby activating E2F transcription factors.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
G1/S-specific cyclin-D1; B-cell lymphoma 1 protein; BCL-1; BCL-1 oncogene; PRAD1 oncogene; CCND1; BCL1; PRAD1
Swiss Prot
P24385
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 250-300 of Human Cyclin D1.
Conjugation
Unconjugated
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 36 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Cyclin D1 (L283) polyclonal antibody detects endogenous levels of Cyclin D1 protein.
Applications Notes
Western blot (WB) analysis of Cyclin D1 (L283) pAb at 1:2000 dilution Lane1:HepG2 whole cell lysate (20ug) Lane2:L02 whole cell lysate (20ug) Lane3:C6 whole cell lysate (20ug) Lane4:3T3-L1 whole cell lysate (20ug)
Host or Source
Rabbit
Available Sizes
Curated Selection
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