Csk (L558) polyclonal antibody

Product Specifications

Background

C terminal Src kinase (CSK) is a non receptor protein tyrosine kinase. One of its major functions is to specifically phosphorylate a conserved C terminal tyrosine of Src family kinases thereby down regulating their kinase activity. CSK is expressed ubiquitously as a protein of approximately 50 KDa in cytosol, but a fraction distributes to lipid rafts due to interaction with the lipid raft associated protein PAG (CSK binding protein) .

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Tyrosine-protein kinase CSK; C-Src kinase; Protein-tyrosine kinase CYL; CSK

Swiss Prot

P41240

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids C-terminus of Human Csk.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 50 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Csk (L558) polyclonal antibody detects endogenous levels of Csk protein.

Applications Notes

Western blot (WB) analysis of Csk (L558) pAb at 1:500 dilution Lane1:PMVEC whole cell lysate (20ug) Lane2:AML-12 whole cell lysate (40ug) Lane3:H1792 whole cell lysate (40ug) Lane4:HepG2 whole cell lysate (40ug)