TPH1 (K54) polyclonal antibody

Product Specifications

Background

Phenylalanine hydroxylase (PAH), tyrosine hydroxylase (TH) and tryptophan hydroxylase (TPH) comprise a small family of monooxygenases that use tetrahydropterine as a cofactor during the catabolism of aromatic L-amino acids. PAH, TH and TPH all contain catalytic domains with an amino-terminal regulatory domain and a short carboxy-terminal tetramerization domain. Each of these enzymes also contains a single ferrous iron atom, which is bound to two histidines and a glutamate and is likely to be involved in the formation of the hydroxylating intermediate. TPH is the first and rate-limiting step in the biosynthesis of serotonin in the central nervous system and melatonin in the pineal gland.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Tryptophan 5-hydroxylase 1; Tryptophan 5-monooxygenase 1; TPH1; TPH; TPRH; TRPH

Swiss Prot

P17752

Reactivity

Human

Immunogen

Synthetic peptide, corresponding to amino acids 20-70 of Human TPH1.

Conjugation

Unconjugated

Applications

WB IHC IF

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 51 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

TPH1 (K54) polyclonal antibody detects endogenous levels of TPH1 protein.

Applications Notes

Western blot (WB) analysis of TPH1 (K54) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate (40ug) Lane2:MCF-7 whole cell lysate (40ug) Lane3:A549 whole cell lysate (40ug)