RPA2 (A17) polyclonal antibody
Product Specifications
Background
RPA is a major damage-recognition protein involved in the early stages of nucleotide excision repair. It can also play a role in telomere maintenance. The C-terminus of RPA 32 can specfically intereact with the DNA repair enzyme UNG2 and repair factors XPA and Rad52, each of which functions in a different repair pathway. In addition, RPA 32 binds specifically to the SH2 domain of Stat3 in vivo, and overexpression of RPA 32 corresponds to the augmented growth factor-stimulated tyrosine phosphorylation and transcription activities of Stat3.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Replication protein A 32 kDa subunit; RP-A p32; RF-A protein 2; RPA2; REPA2; RPA32; RPA34
Swiss Prot
P15927
Reactivity
Human, Mouse
Immunogen
Synthetic peptide, corresponding to the N-terminus of Human RPA2.
Conjugation
Unconjugated
Applications
WB IHC IF
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 32 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
RPA2 (A17) polyclonal antibody detects endogenous levels of RPA2 protein.
Applications Notes
Western blot (WB) analysis of RPA2 (A17) pAb at 1:500 dilution Lane1:H1792 whole cell lysate (40ug) Lane2:EC9706 whole cell lysate (40ug) Lane3:A549 whole cell lysate (40ug) Lane4:SGC7901 whole cell lysate (40ug) Lane5:HCT116 whole cell lysate (40ug) Lane6:PMVEC whole cell lysate (40ug) Lane7:3T3-L1 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
Curated Selection
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