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RPA2 (A17) polyclonal antibody

Product Specifications

Background

RPA is a major damage-recognition protein involved in the early stages of nucleotide excision repair. It can also play a role in telomere maintenance. The C-terminus of RPA 32 can specfically intereact with the DNA repair enzyme UNG2 and repair factors XPA and Rad52, each of which functions in a different repair pathway. In addition, RPA 32 binds specifically to the SH2 domain of Stat3 in vivo, and overexpression of RPA 32 corresponds to the augmented growth factor-stimulated tyrosine phosphorylation and transcription activities of Stat3.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Replication protein A 32 kDa subunit; RP-A p32; RF-A protein 2; RPA2; REPA2; RPA32; RPA34

Swiss Prot

P15927

Reactivity

Human, Mouse

Immunogen

Synthetic peptide, corresponding to the N-terminus of Human RPA2.

Conjugation

Unconjugated

Applications

WB IHC IF

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 32 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

RPA2 (A17) polyclonal antibody detects endogenous levels of RPA2 protein.

Applications Notes

Western blot (WB) analysis of RPA2 (A17) pAb at 1:500 dilution Lane1:H1792 whole cell lysate (40ug) Lane2:EC9706 whole cell lysate (40ug) Lane3:A549 whole cell lysate (40ug) Lane4:SGC7901 whole cell lysate (40ug) Lane5:HCT116 whole cell lysate (40ug) Lane6:PMVEC whole cell lysate (40ug) Lane7:3T3-L1 whole cell lysate (40ug)

Host or Source

Rabbit

Available Sizes

Frequently Asked Questions

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