ErbB-3 (S1285) polyclonal antibody

Product Specifications

Background

The EGF receptor family comprises several related receptor tyrosine kinases that are frequently overexpressed in a variety of carcinomas. Members of this receptor family include EGFR (HER1), Neu (ErbB-2, HER2), ErbB-3 (HER3) and ErbB-4 (HER4), which form either homodimers or heterodimers upon ligand binding. Full length ErbB-3 is overexpressed in human mammary tumors. The ErbB-3 gene also produces several alternative variants, including a secreted form which negatively regulates heregulin stimulated ErbB activation. ErbB-3 heterodimerizes with Neu and binds heregulin in order to activate phosphoinositide (PI) 3-kinase. The recruitment and activation of PI 3-kinase occurs via its interaction with phosphorylated YXXM motifs in the carboxy terminus of ErbB-3.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

ERBB3; ERBB3 protein; erbB3 S; ERBB3; Glial growth factor receptor; HER-3; HER3; LCCS2; MDA BF 1; MGC88033; p180 ErbB3; p45 sErbB3; p85 sErbB3; proto-oncogene-like protein c ErbB 3

Swiss Prot

P21860

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 1250-1300 of Human ErbB-3.

Conjugation

Unconjugated

Related Pathways

ErbB HER Signaling

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 148, 185 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

ErbB-3 (S1285) polyclonal antibody detects endogenous levels of ErbB-3 protein.

Applications Notes

Western blot (WB) analysis of ErbB-3 (S1285) polyclonal antibody at 1:500 dilution Lane1:MCF-7 cell lysate Lane2:Mouse brain tissue lysate Lane3:H9C2 cell lysate