Crystallin-αB (L55) polyclonal antibody

Product Specifications

Background

α-crystallins consist of three gene products, αA, αB and αC-crystallin, which are members of the small heat shock protein family (HSP 20) . They are induced by heat shock, and act as molecular chaperones by holding denatured proteins in large soluble aggregates. However, unlike other molecular chaperones, α-crystallins do not renature these proteins. Expression of αA-crystallin is restricted to the lens. Defects in this gene cause autosomal dominant congenital cataracts (ADCC) . The human αB-crystallin gene product is expressed in many tissues, including lens, heart and skeletal muscle. Elevated expression of αB-crystallin is associated with many neurological diseases, and a missense mutation in this gene has co-segregated in a family with a Desmin-related myopathy.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Alpha-crystallin B chain; Alpha (B) -crystallin; Heat shock protein beta-5; HspB5; Renal carcinoma antigen NY-REN-27; Rosenthal fiber component; CRYAB; CRYA2; αB-Crystallin

Swiss Prot

P02511

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 21-70 of Human Crystallin-αB.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 20 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Crystallin-αB (L55) polyclonal antibody detects endogenous levels of Crystallin-αB protein.

Applications Notes

Western blot (WB) analysis of Crystallin-αB (L55) pAb at 1:500 dilution Lane1:MEF whole cell lysate (40ug) Lane2:PMVEC whole cell lysate (40ug) Lane3:A549 whole cell lysate (40ug) Lane4:HEK293T whole cell lysate (40ug)