Sp1 (G447) polyclonal antibody

Product Specifications

Background

Sp1 is a sequence-specific transcription factor that recognizes GGGGCGGGGC and closely related sequences, which are often referred to as GC boxes. Sp1 was initially identified as a HeLa cell-derived factor that selectively activates in vitro transcription from the SV40 promoter and binds to the multiple GC boxes in the 21 bp repeated elements in SV40. The sequence specificity of DNA binding is conferred by Zn (II) fingers, whereas a different region of Sp1 appears to regulate the affinity of DNA binding. Sp1 belongs to a subgroup of transcription factors that are phosphorylated upon binding to promoter sequences.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Transcription factor Sp1; SP1; TSFP1

Swiss Prot

P08047

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 421-470 of Human Sp1.

Conjugation

Unconjugated

Related Pathways

ESC Pluripotency and Differentiation Signaling Pathway

Applications

WB IHC IF

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 90 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

SP1 (G447) polyclonal antibody detects endogenous levels of SP1 protein.

Applications Notes

Western blot (WB) analysis of Sp1 (G447) polyclonal antibody at 1:500 dilution Lane1:PC12 whole cell lysate (40ug) Lane2:CT26 whole cell lysate (40ug) Lane3:Hela whole cell lysate (40ug) Lane4:COS-7 whole cell lysate (40ug)