SGK 1 (P73) polyclonal antibody

Product Specifications

Background

Serum- and glucocorticoid-regulated kinase (SGK), also known as SGK1, is a serine/threonine protein kinase and a member of the "AGC" subfamily, which includes protein kinases A, G, and C. SGK plays an important role in activating certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. SGK contains a catalytic domain, which is most similar to Akt1. SGK is a downstream target of PI 3-kinase-stimulated growth factor signaling, with 3-phosphoinositide-dependent protein kinase 1 (PDK1) capable of phosphorylating the activation-loop of SGK at Threonine-256.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Serine/threonine protein kinase SGK; Serine/threonine protein kinase Sgk1; Serine/threonine-protein kinase Sgk1; Serum and glucocorticoid regulated kinase; Serum/glucocorticoid regulated kinase 1; Serum/glucocorticoid regulated kinase; Serum/glucocorticoid-regulated kinase 1; SGK 1; SGK; SGK1; SGK1

Swiss Prot

O00141

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 51-100 of Human SGK1.

Conjugation

Unconjugated

Related Pathways

MTOR signaling

Applications

WB IHC IF

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 54 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

SGK (P73) polyclonal antibody detects endogenous levels of SGK protein.

Applications Notes

Western blot (WB) analysis of SGK 1 (P73) pAb at 1:500 dilution Lane1:The Kidney tissue lysate of Mouse (40ug) Lane2:The Kidney tissue lysate of Rat (40ug) Lane3:Panc1 whole cell lysate (40ug) Lane4:A549 whole cell lysate (40ug) Lane5:3T3-L1 whole cell lysate (40ug) Lane6:PMVEC whole cell lysate (40ug) Lane7:HEK293T whole cell lysate (40ug)