IRAK1 (T381) polyclonal antibody
Product Specifications
Background
Three structurally related ligands for IL-1Rs have been described. These include two agonists, IL-1α and IL-1β, and a specific receptor antagonist, IL-1Rα. Two distinct receptors designated IL-1RI and IL-1RII have been identified, each of which belong to the Ig superfamily. The preponderance of evidence suggests IL-1RI to be the functional IL-1 receptor. Binding of IL-1 to its cognate receptor results in the activation of the NFκB signaling pathway. The IL-1-dependent kinase termed IRAK (for IL-1 receptor-associated kinase) co-immunoprecipitates with activated IL-1RI and has been implicated as an upstream mediator of NFκB activation. Additional support for this assertion comes from the fact that a related Drosophila protein, Pelle, is a known upstream activator of Dorsal, the Drosophila homolog of NFκB.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Interleukin-1 receptor-associated kinase 1; IRAK-1; IRAK1; IRAK
Swiss Prot
P51617
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 350-400 of Human IRAK1.
Conjugation
Unconjugated
Related Pathways
Toll-like Receptor signaling
Applications
WB IF
Dilution
WB: 1:500~1:1000 IF: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 76 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
IRAK1 (T381) polyclonal antibody detects endogenous levels of IRAK1 protein.
Applications Notes
Western blot (WB) analysis of IRAK1 (T381) polyclonal antibody at 1:500 dilution Lane1:L02 whole cell lysate (40ug) Lane2:THP-1 whole cell lysate (40ug) Lane3:HCT116 whole cell lysate (40ug) Lane4:PC12 whole cell lysate (40ug) Lane5:CT26 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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