IRAK1 (T381) polyclonal antibody

Three structurally related ligands for IL-1Rs have been described. These include two agonists, IL-1α and IL-1β, and a specific receptor antagonist, IL-1Rα. Two distinct receptors designated IL-1RI and IL-1RII have been identified, each of which belong to the Ig superfamily. The preponderance of evidence suggests IL-1RI to be the functional IL-1 receptor. Binding of IL-1 to its cognate receptor results in the activation of the NFκB signaling pathway. The IL-1-dependent kinase termed IRAK (for IL-1 receptor-associated kinase) co-immunoprecipitates with activated IL-1RI and has been implicated as an upstream mediator of NFκB activation. Additional support for this assertion comes from the fact that a related Drosophila protein, Pelle, is a known upstream activator of Dorsal, the Drosophila homolog of NFκB.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Western blot (WB) analysis of IRAK1 (T381) polyclonal antibody at 1:500 dilution Lane1:L02 whole cell lysate (40ug) Lane2:THP-1 whole cell lysate (40ug) Lane3:HCT116 whole cell lysate (40ug) Lane4:PC12 whole cell lysate (40ug) Lane5:CT26 whole cell lysate (40ug)