XRCC1 (G552) polyclonal antibody

Product Specifications

Background

The x-ray repair cross-complementing (XRCC) proteins are responsible for efficiently repairing and maintaining genetic stability following DNA base damage. These genes share sequence similarity with the yeast DNA repair protein Rad51. XRCC1 is a 70 kDa protein that facilitates the DNA base excision repair pathway by interacting with DNA ligase III and DNA polymerase to repair DNA single-strand breaks. XRCC2 and XRCC3 are both involved in maintaining chromosome stability during cell division. XRCC2 is required for efficient repair of DNA double-strand breaks by homologous recombination between sister chromatids, and XRCC3 interacts directly with Rad51 to cooperate with Rad51 during recombinational repair. XRCC4 is an accessory factor of DNA ligase IV that preferentially binds DNA with nicks or broken ends. XRCC4 binds to DNA ligase IV and enhances its joining activity, and it is also involved in V (D) J recombination.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

XRCC1; X-ray repair cross-complementing protein 1; XRCC1; XRCC1. XRCC 1. XRCC-1. X-ray cross complementing factor X ray. DNA ligase. complementation protein

Swiss Prot

P18887

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 520-570 of Human XRCC1.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 80 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

XRCC1 (G552) polyclonal antibody detects endogenous levels of XRCC1 protein.

Applications Notes

Western blot (WB) analysis of XRCC1 (G552) polyclonal antibody at 1:500 dilution Lane1:HepG2 whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:H9C2 whole cell lysate