VEGFB (R125) polyclonal antibody

Product Specifications

Background

The onset of angiogenesis is believed to be an early event in tumorigenesis and may facilitate tumor progression and metastasis. Several growth factors with angiogenic activity have been described. These include fibroblast growth factor (FGF), platelet derived growth factor (PDGF) and vascular endothelial growth factor (VEGF) . VEGF is a dimeric glycoprotein with struc- tural homology to PDGF. Several variants of VEGF have been described that arise by alternative mRNA splicing. It has been speculated that VEGF may function as a tumor angiogenesis factor in vivo. Two additional proteins des- ignated VEGF-B and VEGF-C share a significant degree of homology withVEGF. VEGF-B is abundantly expressed in heart and skeletal muscle and is frequently co-expressed with VEGF.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Vascular endothelial growth factor B; VEGF-B; VEGF-related factor; VEGFB; VRF

Swiss Prot

P49765

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 100-150 of Human VEGFB.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 22 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

VEGFB (R125) polyclonal antibody detects endogenous levels of VEGFB protein.

Applications Notes

Western blot (WB) analysis of VEGFB (R125) polyclonal antibody at 1:500 dilution Lane1:K562 whole cell lysate (40ug) Lane2:A549 whole cell lysate (40ug) Lane3:The Heart tissue lysate of Rat (40ug) Lane4:The Heart tissue lysate of Mouse (40ug)