Ubiquitin (A46) polyclonal antibody
Product Specifications
Background
Ubiquitin (Ub) is among the most phylogenetically conserved proteins known. The primary function of ubiquitin is to clear abnormal, foreign and improperly folded proteins by targeting them for degradation by the 26S proteosome. This small, 76 amino acid protein can be covalently attached to cellular proteins via an isopeptide linkage between the carboxy terminal group of ubiquitin and lysine amino groups on the acceptor protein. For proteolysis to occur, ubiquitin oligomers must be assembled. Ubiquitin chains on proteolytic substrates are commonly found to have an isopeptide bridge between Lysine 48 of one ubiquitin molecule and the carboxy-terminus of a neighboring ubiquitin molecule.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Polyubiquitin B; RPS 27A; RPS27A; UBA 52; UBA 80; UBA52; UBA80; UBB; UBC; UBCEP 1; UBCEP 2; UBCEP1; UBCEP2; Ubiquitin; ubiquitin B
Swiss Prot
P62988
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 20-60 of Ubiquitin.
Conjugation
Unconjugated
Related Pathways
Ubiquitin Proteasome
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ *8 kDa or Uncertain
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Ubiquitin (A46) polyclonal antibody detects ubiquitin, polyubiquitin and ubiquitinated proteins.
Applications Notes
Western blot (WB) analysis of Ubiquitin (A46) polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate treated with UV Lane2:HEK293T cell ysate Lane3:NIH-3T3 cell lysate treated with UV Lane4:Rat kidney tissue lysate
Host or Source
Rabbit
Available Sizes
Frequently Asked Questions
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