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Ubiquitin (A46) polyclonal antibody

Product Specifications

Background

Ubiquitin (Ub) is among the most phylogenetically conserved proteins known. The primary function of ubiquitin is to clear abnormal, foreign and improperly folded proteins by targeting them for degradation by the 26S proteosome. This small, 76 amino acid protein can be covalently attached to cellular proteins via an isopeptide linkage between the carboxy terminal group of ubiquitin and lysine amino groups on the acceptor protein. For proteolysis to occur, ubiquitin oligomers must be assembled. Ubiquitin chains on proteolytic substrates are commonly found to have an isopeptide bridge between Lysine 48 of one ubiquitin molecule and the carboxy-terminus of a neighboring ubiquitin molecule.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Polyubiquitin B; RPS 27A; RPS27A; UBA 52; UBA 80; UBA52; UBA80; UBB; UBC; UBCEP 1; UBCEP 2; UBCEP1; UBCEP2; Ubiquitin; ubiquitin B

Swiss Prot

P62988

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 20-60 of Ubiquitin.

Conjugation

Unconjugated

Related Pathways

Ubiquitin Proteasome

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ *8 kDa or Uncertain

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Ubiquitin (A46) polyclonal antibody detects ubiquitin, polyubiquitin and ubiquitinated proteins.

Applications Notes

Western blot (WB) analysis of Ubiquitin (A46) polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate treated with UV Lane2:HEK293T cell ysate Lane3:NIH-3T3 cell lysate treated with UV Lane4:Rat kidney tissue lysate

Host or Source

Rabbit

Available Sizes

Frequently Asked Questions

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