SENP5 (L681) polyclonal antibody

Product Specifications

Background

SUMO (small ubiquitin-related modifier), a member of the ubiquitin-like protein family, regulates diverse cellular functions of a variety of target proteins including transcription, DNA repair, nucleocytoplasmic trafficking and chromosome segregation. SUMO precursor proteins undergo cleavage of the residues after the “GG” region by SUMO-specific proteases in maturation. This cleavage of the precursor is a prerequisite for subsequent sumoylation. The sentrin-specific (or SUMO-specific) protease (SENP) proteins belong to the peptidase C48 family and include SENP1-3 and SENP5-8. SENP1, SENP2 and SENP3 degrade UBL1 and SMT3H2 conjugates and subsequently release the monomers from sumoylated substrates.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Sentrin-specific protease 5; Sentrin/SUMO-specific protease SENP5; SENP5; FKSG45

Swiss Prot

Q96HI0

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 650-697 of Human SENP5.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 87 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

SENP5 (L681) polyclonal antibody detects endogenous levels of SENP5 protein.

Applications Notes

Western blot (WB) analysis of SENP5 (L681) pAb at 1:500 dilution Lane1:PC3 whole cell lysate (40ug) Lane2:MCF-7 whole cell lysate (40ug) Lane3:C6 whole cell lysate (40ug) Lane4:MEF whole cell lysate (40ug)