SENP1 (D2) polyclonal antibody

Product Specifications

Background

SUMO (small ubiquitin-related modifier), a member of the ubiquitin-like protein family, regulates diverse cellular functions of a variety of target proteins including transcription, DNA repair, nucleocytoplasmic trafficking and chromosome segregation. SUMO precursor proteins undergo cleavage of the residues after the “GG” region by SUMO-specific proteases in maturation. This cleavage of the precursor is a prerequisite for subsequent sumoylation. The sentrin-specific (or SUMO-specific) protease (SENP) proteins belong to the peptidase C48 family and include SENP1-3 and SENP5-8. SENP1, SENP2 and SENP3 degrade UBL1 and SMT3H2 conjugates and subsequently release the monomers from sumoylated substrates.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Sentrin-specific protease 1; Sentrin/SUMO-specific protease SENP1; SEN1

Swiss Prot

Q9P0U3

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to the N-terminual of Human SENP1.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 73 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

SENP1 (D2) polyclonal antibody detects endogenous levels of SENP1 protein.

Applications Notes

Western blot (WB) analysis of SENP1 (D2) polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate (40ug) Lane2:The Testis cells tissue lysate of Rat (40ug) Lane3:The Testis cells tissue lysate of Mouse (40ug)