PIAS 1 (G24) polyclonal antibody
Product Specifications
Background
The IL-6-type family of cytokines, which includes IL-6 as well as a number of similar cytokines and growth factors, plays a significant role in regulating gene activation, proliferation and differentiation. Transcription factors of the Stat family are known to be involved in this signal transduction pathway, undergoing phosphorylation, dimerization and translocation to the nucleus upon activation. PIAS 1, for protein inhibitor of activated Stat1 (also designated Gu/RNA helicase II binding protein), binds specifically to Stat1, blocking Stat1 DNA-binding activity and inhibiting Stat1-mediated gene activation. PIAS 1 also binds to the Gu/RNA helicase II enzyme, leading to the proteolytic cleavage of Gu/RH-II. PIAS 3 similarly binds specifically to Stat3, blocking Stat3 DNA-binding activity and inhibiting Stat3-mediated gene activation.
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
E3 SUMO-protein ligase PIAS1; Protein inhibitor of activated STAT protein 1; Gu binding protein; GBP; RNA helicase II binding protein; DEAD/H box-binding protein 1; DDXBP1
Swiss Prot
O75925
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 1-50 of Human PIAS 1.
Conjugation
Unconjugated
Related Pathways
Jak Stat : IL-6 Receptor Signaling, Examples of Crosstalk Between Post-translational Modifications
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 72 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
PIAS1 (G24) polyclonal antibody detects endogenous levels of PIAS1 protein.
Applications Notes
Western blot (WB) analysis of PIAS 1 (G24) polyclonal antibody at 1:500 dilution Lane1:MCF-7 whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:H9C2 whole cell lysate
Host or Source
Rabbit
CAS Number
9007-83-4
Available Sizes
Curated Selection
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